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Title 

Euphorbia supina inhibits inflammatory mediators in mouse bone marrow-derived mast cells and macrophages

Authors 

H S ChaeHyuk-Hwan SongY M KimHyeong Kyu LeeSei-Ryang OhY W Chin

Publisher 

Elsevier

Issue Date 

2015

Citation 

International Immunopharmacology, vol. 29, no. 2, pp. 966-973

Keywords 

5-LipoxygenaseEdemaEuphorbia supinaInducible nitric oxide synthaseInflammationInterleukin-6

Abstract 

Euphorbia supina has been traditionally used for the treatment of furuncle and bloody diarrhea relevant to the inflammatory process. It has been proven to have a variety of pharmacological efficacies including antiarthritic, detoxification, hemostatic, and diuretic activities. RAW 264.7 macrophages and bone marrow-derived mast cells (BMMCs) were used to determine the anti-inflammatory and anti-allergic effects of E. supina (ES). NO production was assayed by measuring the nitrite content of the supernatants of cultured RAW 264.7 cells. β-hexosaminidase, a marker of mast cell degranulation, was quantitated by spectrophotometric analysis. ELISA was used for the analysis of interleukin-6 expression, and Western blotting was used to analyze 5-LOX, iNOS, and MAPK activation. The relevant gene expression upon ES treatment was measured by RT-PCR. ES inhibited inducible nitric oxide synthase (iNOS) in RAW 264.7 cells, and IL-6 and LTC4 production in PMA- and A23187-induced BMMCs along with the downregulation of 5-LOX gene expression. Furthermore, in the present study, a decrease in p-ERK, p-JNK, and p-P38 expression, as well as the suppression of degranulation, were observed by treatment with ES. Further in vivo study revealed that ES treatment also remarkably inhibited xylene-induced mouse ear edema and MPO levels in mice ears. This study demonstrates that ES has a potential regulatory effect on the expression of inflammatory mediators through the inhibition of both the phosphorylation of MAPK signaling and the activation of degranulation.

ISSN 

1567-5769

Link 

http://dx.doi.org/10.1016/j.intimp.2015.09.008

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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