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Title 

Bacterial RNAs activate innate immunity in Arabidopsis

Authors 

Boyoung LeeYong-Soon ParkSoohyun LeeGeun Cheol SongChoong-Min Ryu

Publisher 

Wiley-Blackwell

Issue Date 

2016

Citation 

New Phytologist, vol. 209, no. 2, pp. 785-797

Keywords 

Pseudomonas syringaeBacterial RNAsJasmonic acid (JA)Microbe-associated molecular pattern (MAMP)Plant innate immunitySalicylic acid (SA)

Abstract 

The common molecular patterns of microbes play a critical role in the regulation of plant innate immunity. However, little is known about the role of nucleic acids in this process in plants. We pre-infiltrated Arabidopsis leaves with total RNAs from Pseudomonas syringae pv. tomato DC3000 (Pto DC3000) and subsequently inoculated these plants with the same bacterial cells. Total Pto DC3000 RNAs pre-infiltrated into Arabidopsis leaves elicited plant immune responses against Pto DC3000. However, sheared RNAs and RNase A application failed to induce immunity, suggesting that intact bacterial RNAs function in plant innate immunity. This notion was supported by the positive regulation of superoxide anion levels, callose deposition, two mitogen-activated protein kinases and defense-related genes observed in bacterial RNA-pre-treated leaves. Intriguingly, the Pto DC3000 population was not compromised in known pattern recognition receptor mutants for chitin, flagellin and elongation factor-Tu (EF-Tu). Plant defense-related mutant analyses further revealed that bacterial RNA-elicited innate immunity was normally required for salicylic and jasmonic acid signaling. Notably, among total RNAs, the abundant bacterial RNA species 16S and 23S ribosomal RNAs were the major determinants of this response. Our findings provide evidence that bacterial RNA serves as a microbe-associated molecular pattern in plants.

ISSN 

0028-646X

Link 

http://dx.doi.org/10.1111/nph.13717.

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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