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Title | Enzymatic synthesis of novel isobavachalcone glucosides via a UDP-glycosyltransferase |
Authors | H M Li; Jae Kyung Lee; L J Nie; Q Huo; T Ma; J K Sohng; Young-Soo Hong; C Z Wu |
Publisher | Pharmaceutical Society of Korea |
Issue Date | 2015 |
Citation | Archives of Pharmacal Research, vol. 38, no. 12, pp. 2208-2215 |
Keywords | Cytotoxicity; Diversification; Glycosylation; Isobavachalcone; YjiC |
Abstract | Glycosylation is often used to improve a natural product's properties such as water solubility, chemical stability, pharmacological potency, and structure diversification. In this study, we studied the enzymatic synthesis of novel isobavachalcone glucosides using a UDP-glycosyltransferase (YjiC) from Bacillus licheniformis DSM-13. The chemical structures of compounds 1 and 2 were elucidated by spectroscopic techniques, including LC-MS, MS, and NMR. Meanwhile, the parameters of glycosylation reaction such as incubation time, UDP-glucose concentration, and pH of buffer were also optimized during this study. Furthermore, the compounds 1 and 2 exhibited weak anti-proliferative activities against five human cancer cell lines, with IC50 values ranging from 58.6 to 86.6 μM. |
ISSN | 0253-6269 |
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Appears in Collections | |
Registered Date |
2019-05-02 |
