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Title 

Transcriptomic features of bovine blastocysts derived by somatic cell nuclear transfer

Authors 

ByungKuk MinSunwha ChoJung Sun ParkYun-Gyeong LeeNamshin KimYong-Kook Kang

Publisher 

Genetics Society of America

Issue Date 

2015

Citation 

G3, vol. 5, no. 12, pp. 2527-2538

Keywords 

Bovine blastocystDevelopmentRNA-seqSCNTTranscriptomes

Abstract 

Reprogramming incompletely occurs in most somatic cell nuclear transfer (SCNT) embryos, which results in misregulation of developmentally important genes and subsequent embryonic malfunction and lethality. Here we examined transcriptome profiles in single bovine blastocysts derived by in vitro fertilization (IVF) and SCNT. Different types of donor cells, cumulus cell and ear-skin fibroblast, were used to derive cSCNT and fSCNT blastocysts, respectively. SCNT blastocysts expressed 13,606 genes on average, similar to IVF (13,542). Correlation analysis found that both cSCNT and fSCNT blastocyst groups had transcriptomic features distinctive from the IVF group, with the cSCNT transcriptomes closer to the IVF ones than the fSCNT. Gene expression analysis identified 56 underrepresented and 78 overrepresented differentially expressed genes in both SCNT groups. A 400-kb locus harboring zinc-finger protein family genes in chromosome 18 were found coordinately down-regulated in fSCNT blastocysts, showing a feature of reprogramming-resistant regions. Probing into different categories of genes important for blastocyst development revealed that genes involved in trophectoderm development frequently were underrepresented, and those encoding epigenetic modifiers tended to be overrepresented in SCNT blastocysts. Our effort to identify reprogramming-resistant, differentially expressed genes can help map reprogramming error-prone loci onto the genome and elucidate how to handle the stochastic events of reprogramming to improve cloning efficiency.

ISSN 

2160-1836

Link 

http://dx.doi.org/10.1534/g3.115.020016

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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