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Title 

Effect of hydroxysafflor yellow A on tyrosinase: Integration of inhibition kinetics with computational simulation

Authors 

S J YinK Y LiuJinhyuk LeeJ M YangG Y QianY X SiY D Park

Publisher 

Elsevier

Issue Date 

2015

Citation 

Process Biochemistry, vol. 50, no. 12, pp. 2112-2120

Keywords 

Docking simulationHydroxysafflor yellow AInhibition kineticsMolecular dynamicsaTyrosinase

Abstract 

Hydroxysafflor yellow A (HSYA) is found in the flower of Carthamus tinctorius. We evaluated the effects of hydroxysafflor yellow A (HSYA) on tyrosinase kinetics and computationally simulated the interaction between HSYA and tyrosinase. HSYA was found to be a mixed-type, reversible inhibitor of tyrosinase, with a Ki of 0.285 ± 0.040 mM. Kinetic measurements of intrinsic and ANS-binding fluorescence showed that HSYA induced changes in the tertiary structure of tyrosinase. HSYA showed a relatively strong binding affinity for tyrosinase and one possible binding site was identified. To gain further insight into the HSYA/tyrosinase interaction, we performed computational docking and molecular dynamics simulations. These experiments indicated that HSYA can interact with several residues near the tyrosinase active site. Our study provides insight into the mechanism by which chalcone compounds such as flavonoids inhibit tyrosinase. Since HSYA inhibits tyrosinase and is also an antioxidant, HSYA is a potential natural antipigmentation agent.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/j.procbio.2015.09.020

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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