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Title 

Development of a highly specific and sensitive cadmium and lead microbial biosensor using synthetic CadC-T7 genetic circuitry

Authors 

Hyun Ju KimJ W LimHaeyoung JeongS J LeeD W LeeT KimSang Jun Lee

Publisher 

Elsevier

Issue Date 

2016

Citation 

Biosensors & Bioelectronics, vol. 79, no. 0, pp. 701-708

Keywords 

CadCHeavy metal ionsMicrobial biosensorMicrofluidic deviceT7 RNA polymerase

Abstract 

Multiple copies of a cadC homolog encoding a heavy metal-responsive transcription factor were found in the genome of a bacterium isolated from ocean sediment, and the heavy metal responses of the encoded proteins were characterized using a fluorescence reporter assay. Each CadC regulator exhibited distinct specificity in response to heavy metal ions, indicating their potential use as modular heavy metal biosensors. Next, we constructed CadC-controlled T7 RNA transcription systems for intracellular signal amplification, i.e., higher sensitivity. Flow cytometry revealed that cadmium and lead ions could be recognized specifically by CadC-T7 biosensors, which could be combined with a microfluidic platform to generate heavy metal biosensor devices with increased sensitivity. Our results demonstrate the successful development of synthetic CadC-T7 genetic circuitry for use in improved heavy metal biosensor microfluidic devices.

ISSN 

0956-5663

Link 

http://dx.doi.org/10.1016/j.bios.2015.12.101

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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