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Title 

Combinatorial application of two aldehyde oxidoreductases on isobutanol production in the presence of furfural

Authors 

H M SeoJ M JeonJ H LeeH S SongH B JooS H ParkK Y ChoiYong Hyun KimK ParkJungoh AhnHong-Weon LeeY H Yang

Publisher 

Springer

Issue Date 

2016

Citation 

Journal of Industrial Microbiology (& Biotechnology), vol. 43, no. 1, pp. 37-44

Keywords 

Aldehyde oxidoreductaseEscherichia coliFurfuralIsobutanol

Abstract 

Furfural is a toxic by-product formulated from pretreatment processes of lignocellulosic biomass. In order to utilize the lignocellulosic biomass on isobutanol production, inhibitory effect of the furfural on isobutanol production was investigated and combinatorial application of two oxidoreductases, FucO and YqhD, was suggested as an alternative strategy. Furfural decreased cell growth and isobutanol production when only YqhD or FucO was employed as an isobutyraldehyde oxidoreductase. However, combinatorial overexpression of FucO and YqhD could overcome the inhibitory effect of furfural giving higher isobutanol production by 110 % compared with overexpression of YqhD. The combinatorial oxidoreductases increased furfural detoxification rate 2.1-fold and also accelerated glucose consumption 1.4-fold. When it compares to another known system increasing furfural tolerance, membrane-bound transhydrogenase (pntAB), the combinatorial aldehyde oxidoreductases were better on cell growth and production. Thus, to control oxidoreductases is important to produce isobutanol using furfural-containing biomass and the combinatorial overexpression of FucO and YqhD can be an alternative strategy.

ISSN 

0169-4146

Link 

http://dx.doi.org/10.1007/s10295-015-1718-2

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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