상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Macaca specific exon creation event generates a novel ZKSCAN5 transcript

Authors 

Young-Hyun KimSe Hee ChoeBong-Seok SongSang Je ParkMyung Jin KimYoung-Ho ParkSeung Bin YoonYoungjeon LeeYeung Bae JinBo Woong SimJi-Su KimKang Jin JeongSun-Uk KimSang-Rae LeeY I ParkJae Won HuhKyu Tae Chang

Publisher 

Elsevier

Issue Date 

2016

Citation 

Gene, vol. 577, no. 2, pp. 236-243

Keywords 

Alternative splicingExon creationLineage specificMacacaZKSCAN5

Abstract 

ZKSCAN5 (also known as ZFP95) is a zinc-finger protein belonging to the Kruppel family. ZKSCAN5 contains a SCAN box and a KRAB A domain and is proposed to play a distinct role during spermatogenesis. In humans, alternatively spliced ZKSCAN5 transcripts with different 5'-untranslated regions (. UTRs) have been identified. However, investigation of our Macaca UniGene Database revealed novel alternative ZKSCAN5 transcripts that arose due to an exon creation event. Therefore, in this study, we identified the full-length sequences of ZKSCAN5 and its alternative transcripts in Macaca spp. Additionally, we investigated different nonhuman primate sequences to elucidate the molecular mechanism underlying the exon creation event. We analyzed the evolutionary features of the ZKSCAN5 transcripts by reverse transcription polymerase chain reaction (RT-PCR) and genomic PCR, and by sequencing various nonhuman primate DNA and RNA samples. The exon-created transcript was only detected in the Macaca lineage (crab-eating monkey and rhesus monkey). Full-length sequence analysis by rapid amplification of cDNA ends (RACE) identified ten full-length transcripts and four functional isoforms of ZKSCAN5. Protein sequence analyses revealed the presence of two groups of isoforms that arose because of differences in start-codon usage. Together, our results demonstrate that there has been specific selection for a discrete set of ZKSCAN5 variants in the Macaca lineage. Furthermore, study of this locus (and perhaps others) in Macaca spp. might facilitate our understanding of the evolutionary pressures that have shaped the mechanism of exon creation in primates.

ISSN 

0378-1119

Link 

http://dx.doi.org/10.1016/j.gene.2015.11.051

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)