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Title 

Inhibitory effect of withaferin A on Helicobacter pylori-induced IL-8 production and NF-κB activation in gastric epithelial cells

Authors 

G KimT H KimM J KangJ A ChoiD Y PackI R LeeM G KimS S HanBo Yeon KimS M OhK B LeeD J KimJ H Park

Publisher 

Spandidos Publications

Issue Date 

2016

Citation 

Molecular Medicine Reports, vol. 13, no. 1, pp. 967-972

Keywords 

Gastric epithelial cellsHelicobacter pyloriInterleukin-8Nuclear factor-κBWithaferin A

Abstract 

Withaferin A (WA), a withanolide purified from Withania somnifera, has been known to exert anti-inflammatory effects. The present study sought to determine the effects of WA on Helicobacter (H.) pylori-mediated inflammation in the AGS gastric epithelial cell line. Cellular production of interleukin (IL)-8 and vascular endothelial growth factor (VEGF) was measured by ELISA. Western blot analysis was performed to determine the activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) as well as hypoxia-inducible factor 1α stabilization. Bacterial growth was also examined by measuring the optical density. Pre-treatment or co-treatment with WA efficiently reduced IL-8 production by AGS cells in response to H. Pylori infection. H. Pylori-induced activation of NF-κB, but not MAPKs, was also inhibited by pre-treatment of WA in the cells. However, WA did not affect VEGF production and HIF-1α stabilization induced by H. Pylori in AGS cells. In addition, WA did not influence the growth of H. Pylori, suggesting that the anti-inflammatory effect of WA was not due to any bactericidal effect. These findings indicate that WA is a potential preventive or therapeutic agent for H. Pylori-mediated gastric inflammation.

ISSN 

1791-2997

Link 

http://dx.doi.org/10.3892/mmr.2015.4602

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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