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Title 

Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus

Authors 

N SuzukiN KishineZ FujimotoM SakuraiM MommaJin-A KoS H NamA KimuraY M Kim

Publisher 

Oxford University Press

Issue Date 

2016

Citation 

Journal of Biochemistry, vol. 159, no. 3, pp. 331-339

Keywords 

dextranaseenzyme-substrate complexglycoside hydrolase family 66Thermoanaereobacterthermostability

Abstract 

The crystal structures of the wild type and catalytic mutant Asp-312→Gly in complex with isomaltohexaose of endo-1,6-dextranase from the thermophilic bacterium Thermoanaerobacter pseudethanolicus (TpDex), belonging to the glycoside hydrolase family 66, were determined. TpDex consists of three structural domains, a catalytic domain comprising an (β/α)8-barrel and two β-domains located at both N- and C-terminal ends. The isomaltohexaose-complex structure demonstrated that the isomaltohexaose molecule was bound across the catalytic site, showing that TpDex had six subsites (-4 to +2) in the catalytic cleft. Marked movement of the Trp-376 side-chain along with loop 6, which was the side wall component of the cleft at subsite +1, was observed to occupy subsite +1, indicating that it might expel the cleaved aglycone subsite after the hydrolysis reaction. Structural comparison with other mesophilic enzymes indicated that several structural features of TpDex, loop deletion, salt bridge and surface-exposed charged residue, may contribute to thermostability.

ISSN 

0021-924X

Link 

http://dx.doi.org/10.1093/jb/mvv104

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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