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Title 

Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans

Authors 

Ji-Yeon KangOhsuk KwonJin Young GilDoo-Byoung Oh

Publisher 

Elsevier

Issue Date 

2016

Citation 

Analytical Biochemistry, vol. 501, no. 0, pp. 1-3

Keywords 

Fluorescent labeling tagGlycan analysisHigh-performance liquid chromatography (HPLC)Mannose-6-phosphate (M-6-P)Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry

Abstract 

Mannose-6-phosphate (M-6-P) glycan analysis is important for quality control of therapeutic enzymes for lysosomal storage diseases. Here, we found that the analysis of glycans containing two M-6-Ps was highly affected by the hydrophilicity of the elution solvent used in high-performance liquid chromatography (HPLC). In addition, the performances of three fluorescent tags - 2-aminobenzoic acid (2-AA), 2-aminobenzamide (2-AB), and 3-(acetyl-amino)-6-aminoacridine (AA-Ac) - were compared with each other for M-6-P glycan analysis using HPLC and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The best performance for analyzing M-6-P glycans was shown by 2-AA labeling in both analyses.

ISSN 

0003-2697

Link 

http://dx.doi.org/10.1016/j.ab.2016.02.004

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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