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Title 

The HIST1 locus escapes reprogramming in cloned bovine embryos

Authors 

ByungKuk MinSunwha ChoJung Sun ParkKyuheum JeonYong-Kook Kang

Publisher 

Genetics Society of America

Issue Date 

2016

Citation 

G3, vol. 6, no. 5, pp. 1365-1371

Keywords 

DNA methylationEpigeneticsHDAC inhibitorHIST1 clusterReprogrammingRNA-seqSCNT

Abstract 

Epigenetic reprogramming is necessary in somatic cell nuclear transfer (SCNT) embryos in order to erase the differentiation-associated epigenetic marks of donor cells. However, such epigenetic memories often persist throughout the course of clonal development, thus decreasing cloning efficiency. Here, we explored reprogramming-refractory regions in bovine SCNT blastocyst transcriptomes. We observed that histone genes residing in the 1.5 Mb spanning the cow HIST1 cluster were coordinately downregulated in SCNT blastocysts. In contrast, both the nonhistone genes of this cluster, and histone genes elsewhere remained unaffected. This indicated that the downregulation was specific to HIST1 histone genes. We found that, after trichostatin A treatment, HIST1 histone genes were derepressed, and DNA methylation at their promoters was decreased to the level of in vitro fertilization embryos. Therefore, our results indicate that the reduced expression of HIST1 histone genes is a consequence of poor epigenetic reprogramming in SCNT blastocysts.

ISSN 

2160-1836

Link 

http://dx.doi.org/10.1534/g3.115.026666

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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