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Title 

Differential matrix metalloprotease (MMP) expression profiles found in aged gingiva

Authors 

S KimS H AhnJ S LeeJ E SongS H ChoS JungSeon-Kyu KimSeok Ho KimKwang-Pyo LeeKi Sun KwonT H Lee

Publisher 

Public Library of Science

Issue Date 

2016

Citation 

Plos One, vol. 11, no. 7, pp. e0158777-e0158777

Abstract 

The periodontium undergoes age-related cellular and clinical changes, but the involved genes are not yet known. Here, we investigated age-related genetic changes in gingiva at the transcriptomic level. Genes that were differentially expressed between young and old human gingiva were identified by RNA sequencing and verified by real-time PCR. A total of 1939 mRNA transcripts showed significantly differential expression between young and old gingival tissues. Matrix metalloprotease (MMP) regulation was the top pathway involved in gingival aging. MMP3, MMP9, MMP12, and MMP13 were upregulated in old gingival tissues, concomitantly with interleukin-1 beta (IL1B) expression. In vitro experiments using human gingival fibroblasts (hGFs) showed that MMP12 was upregulated in old hGFs compared to young hGFs. Moreover, the MMP3, MMP9 and IL1B levels were more highly stimulated by infection with the oral bacterium, Fusobacterium nucleatum, in old hGFs compared to young hGFs. Collectively, these findings suggest that, in gingiva, the upregulation of MMP12 may be a molecular hallmark of natural aging, while the upregulations of MMP3, MMM9, and IL1B may indicate externally (e.g., infection)-induced aging. These findings contribute to our understanding of the molecular targets involved in gingival aging.

ISSN 

1932-6203

Link 

http://dx.doi.org/10.1371/journal.pone.0158777

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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