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Title 

Regional differences of proteins expressing in Adipose depots isolated from cows, steers and bulls as identified by a proteomic approach

Authors 

J H ChoJ Y JeongR H LeeM N ParkSeok Ho KimS M ParkJ C ShinY J JeonJ H ShimN J ChoiK S SeoY S ChoM S KimS KoJ M SeoS Y LeeJ I ChaeH J Lee

Publisher 

The Asian-australasian Association of Animal

Issue Date 

2016

Citation 

Asian-Australasian Journal of Animal Sciences, vol. 29, no. 8, pp. 1197-1206

Keywords 

Adipose depotsHanwooLiquid chromatography-tandem mass spectrometryMarblingMeat quality

Abstract 

Adipose tissue in the loin muscle area of beef cattle as a marbling factor is directly associated with beef quality. To elucidate whether properties of proteins involved in depot specific adipose tissue were sex-dependent, we analyzed protein expression of intramuscular adipose tissue (IMAT) and omental adipose tissue (OMAT) from Hanwoo cows, steers, and bulls of Korean native beef cattle by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis, quantitative polymerase chain reaction (PCR) and western blot analysis. Two different adipose depots (i.e. intramuscular and omental) were collected from cows (n = 7), steers (n = 7), or bulls (n = 7). LC-MS/MS revealed a total of 55 and 35 proteins in IMAT and OMAT, respectively. Of the 55 proteins identified, 44, 40, and 42 proteins were confirmed to be differentially expressed in IMAT of cows, steers, and bulls, respectively. In OMAT of cows, steers, and bulls, 33, 33, and 22 were confirmed to be differentially expressed, respectively. Tropomyosin (TPM) 1, TPM 2, and TPM3 were subjected to verification by quantitative PCR and western blot analysis in IMAT and OMAT of Hanwoo cows, steers, and bulls as key factors closely associated with muscle development. Both mRNA levels and protein levels of TPM1, TPM2, and TPM3 in IMAT were lower in bulls compared to in cows or steers suggesting that they were positively correlated with marbling score and quality grade. Our results may aid the regulation of marbling development and improvement of meat quality grades in beef cattle.

ISSN 

1011-2367

Link 

http://dx.doi.org/10.5713/ajas.16.0051Document

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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