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Title 

Lipase-catalyzed in-situ biosynthesis of glycerol-free biodiesel from heterotrophic microalgae, Aurantiochytrium sp. KRS101 biomass

Authors 

K H KimO K LeeChul Ho KimJeong-Woo SeoBaek Rock OhE Y Lee

Publisher 

Elsevier

Issue Date 

2016

Citation 

Bioresource Technology, vol. 211, no. 0, pp. 472-477

Keywords 

Aurantiochytrium sp. KRS101BiodieselFatty acid methyl estersHeterotrophic microalgaeIn-situ esterification

Abstract 

Heterotrophic microalgae, Aurantiochytrium sp. KRS101 had a large amount of lipid (56.8% total lipids). The cells in the culture medium were easily ruptured due to thin cell wall of Aurantiochytrium sp., which facilitated in-situ fatty acid methyl esters (FAMEs) production directly from biomass. The harvested biomass had a high content of free fatty acids (FFAs), which was advantageous for glycerol-free FAMEs production. FAMEs were directly produced from Aurantiochytrium sp. KRS101 biomass (48.4% saponifiable lipids) using Novozyme 435-catalyzed in-situ esterification in dimethyl carbonate (DMC). DMC was used as a lipid extraction reagent, acyl acceptor and reaction medium. A 433.09 mg FAMEs/g biomass was obtained with 89.5% conversion under the optimal condition: DMC to biomass ratio of 5:1 (v/w) and enzyme to biomass ratio of 30% (w/w) at 50 °C for 12 h. Glycerol could not be detected in the produced FAMEs.

ISSN 

0960-8524

Link 

http://dx.doi.org/10.1016/j.biortech.2016.03.092

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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