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Title 

BaeR protein acts as an activator of nuclear factor-kappa B and Janus kinase 2 to induce inflammation in murine cell lines

Authors 

S J LeeB T BirhanuE G AwjiMyung Hee KimJ Y ParkJ W SuhS C Park

Publisher 

National Research Council Canada

Issue Date 

2016

Citation 

Canadian Journal of Microbiology, vol. 62, no. 9, pp. 753-761

Keywords 

BaeRJAK2NF-κBNitric oxideSalmonella enterica Paratyphi A

Abstract 

BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogenactivated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to induce an inflammatory response. BaeR protein was purified from Salmonella enterica Paratyphi A and subcloned into a pPosKJ expression vector. RAW 264.7 cells were treated with BaeR, and RNA was extracted by TRIzol reagent for RT-PCR. Cytokine gene expression was analyzed by using the comparative cycle threshold method, while western blotting and ELISA were used to assess protein expression. We confirmed that BaeR activates nuclear factor-kappa B (NF-κB), thereby inducing an inflammatory response and increases the production of interleukins (IL-)1β and IL-6. During this process, the Janus kinase 2 (JAK2)-STAT1 signaling pathway was activated, resulting in an increase in the release of interferons I and II. Additionally, COX-2 was activated and its expression increased with time. In conclusion, BaeR induced an inflammatory response through activation of NF-κB in addition to the MAPKs. Furthermore, activation of the JAK2-STAT1 pathway and COX-2 facilitated the cytokine binding activity, suggesting an additional role for BaeR in the modulation of the immune system of the host and the virulence activity of the pathogen.

ISSN 

0008-4166

Link 

http://dx.doi.org/10.1139/cjm-2016-0057

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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