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Title 

Production of transgenic cucumber expressing phytoene synthase-2A carotene desaturase gene

Authors 

Hyun A JangS D UtomoSuk Yoon KwonS H HaY Xing-guoP S Choi

Publisher 

Korea Society of Plant Biotechnology

Issue Date 

2016

Citation 

Journal of Plant Biotechnology, vol. 43, no. 3, pp. 341-346

Keywords 

Cotyledonary-node explantCucumis sativusTransformation efficiencyβ-carotene

Abstract 

The objectives of this study were to 1) evaluate the efficiency of the protocol of Agrobacterium-mediated transformation of cucumber to introduce phytoene synthase-2a carotene desaturase (PAC genes); 2) demonstrate the integration of PAC genes into the genome of putative transgenic cucumber based on growth on selection medium, PCR and Southern analysis; 3) evaluate the expression of PAC genes in transgenic cucumber based on the analysis of RT-PCR and Northern blot hybridization. Out of 5,945 cotyledonary-node explants inoculated with Agrobacterium, 65 (1.1%) explants produced 238 shoots. Integration of PAC genes into the genome of the cucumber was demonstrated based on the analysis of gDNA-PCR, 21 out of the 238 plants regenerated; while 6 plants proved positive for Southern blot hybridization. Transgene expression was demonstrated based on analysis of RT-PCR, 6 plants proved positive out of the 6 plants analyzed; while 4 plants out of 6 proved positive during Northern blot hybridization. This study successfully demonst rated t he production of transgenic cucumber, integration, and expression of the PAC gene in cucumber.

ISSN 

1229-2818

Link 

http://dx.doi.org/10.5010/JPB.2016.43.3.341

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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