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Title 

Histone H4 is cleaved by granzyme A during staurosporine-induced cell death in B-lymphoid Raji cells

Authors 

Phil Young LeeByoung Chul ParkSeung-Wook ChiKwang-Hee BaeSunhong KimS ChoS KangJeong Hoon KimSung Goo Park

Publisher 

Korean Society for Biochemistry and Molecular Biology

Issue Date 

2016

Citation 

BMB Reports, vol. 49, no. 10, pp. 560-565

Keywords 

Caspase-independent cell deathGranzyme AHistone H4Raji cell

Abstract 

Granzyme A (GzmA) was first identified as a cytotoxic T lymphocyte protease protein with limited tissue expression. A number of cellular proteins are known to be cleaved by GzmA, and its function is to induce apoptosis. Histones H1, H2B, and H3 were identified as GzmA substrates during apoptotic cell death. Here, we demonstrated that histone H4 was cleaved by GzmA during staurosporine-induced cell death; however, in the presence of caspase inhibitors, staurosporine-treated Raji cells underwent necroptosis instead of apoptosis. Furthermore, histone H4 cleavage was blocked by the GzmA inhibitor nafamostat mesylate and by GzmA knockdown using siRNA. These results suggest that histone H4 is a novel substrate for GzmA in staurosporine-induced cells.

ISSN 

1225-8687

Link 

http://dx.doi.org/10.5483/BMBRep.2016.49.http://dx.doi.org/10.105

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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