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Title 

Direct protein?protein conjugation by genetically introducing bioorthogonal functional groups into proteins

Authors 

S KimW KoBong Hyun SungS C KimH S Lee

Publisher 

Elsevier

Issue Date 

2016

Citation 

Bioorganic & Medicinal Chemistry, vol. 24, no. 22, pp. 5816-5822

Keywords 

Click reactionGenetic incorporationProtein?protein conjugationUnnatural amino acids

Abstract 

Proteins often function as complex structures in conjunction with other proteins. Because these complex structures are essential for sophisticated functions, developing protein?protein conjugates has gained research interest. In this study, site-specific protein?protein conjugation was performed by genetically incorporating an azide-containing amino acid into one protein and a bicyclononyne (BCN)-containing amino acid into the other. Three to four sites in each of the proteins were tested for conjugation efficiency, and three combinations showed excellent conjugation efficiency. The genetic incorporation of unnatural amino acids (UAAs) is technically simple and produces the mutant protein in high yield. In addition, the conjugation reaction can be conducted by simple mixing, and does not require additional reagents or linker molecules. Therefore, this method may prove very useful for generating protein?protein conjugates and protein complexes of biochemical significance

ISSN 

0968-0896

Link 

http://dx.doi.org/10.1016/j.bmc.2016.09.035

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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