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Title 

Codon optimization of Saccharomyces cerevisiae mating factor alpha prepro-leader to improve recombinant protein production in Pichia pastoris

 

피키아 파스토리스에의 재조합 단백질 생산 향상을 위한 사카로마이세스 유래의 분비서열 코돈 최적화

Authors 

Jungoh AhnMin-Jung JangK S AngHong-Weon LeeEui Sung ChoiD Y Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

2016

Citation 

Biotechnology Letters, vol. 38, no. 12, pp. 2137-2143

Keywords 

Codon optimizationCodon-pair contextIndividual codon usageMating factor α prepro-leader sequencePichia pastorisSaccharomyces cerevisiaeSynthetic biology

Abstract 

Objectives: To evaluate different codon optimization parameters on the Saccharomyces cerevisiae-derived mating factor α prepro-leader sequence (MFLS) to improve Candida antarctica lipase B (CAL-B) secretory production in Pichia pastoris. Results: Codon optimization based on the individual codon usage (ICU) and codon context (CC) design parameters enhanced secretory production of CAL-B to 7?U/ml and 12?U/ml, respectively. Only 3?U/ml was obtained with the wild type sequence while the sequence optimized using both ICU and CC objectives showed intermediate performance of 10?U/ml. These results clearly show that CC is the most relevant parameter for the codon optimization of MFLS in P. pastoris, and there is no synergistic effect achieved by considering both ICU and CC together. Conclusion: The CC optimized MFLS increased secretory protein production of CAL-B in P. pastoris by fourfold.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-016-2203-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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