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Title 

Farnesol production in Escherichia coli through the construction of a farnesol biosynthesis pathway ? application of PgpB and YbjG phosphatases

 

파네솔 생합성 경로 구축을 통한 대장균에서의 파네솔 생산 - PgpB 및 YbjG의 활용

Authors 

C WangJ E ParkEui Sung ChoiS W Kim

Publisher 

Wiley-Blackwell

Issue Date 

2016

Citation 

Biotechnology Journal, vol. 11, no. 0, pp. 1291-1297

Keywords 

Escherichia coliFarnesolPgpBPhosphataseYbjG

Abstract 

Farnesol is a sesquiterpenoid alcohol that has important industrial and medical potential. It is usually synthesized from farnesyl diphosphate (FPP) by farnesol synthase in plants. FPP accumulation can cause up-regulation of phosphatases capable of FPP hydrolysis, resulting in farnesol production in Escherichia coli. We found that PgpB and YbjG, two integral membrane phosphatases, can hydrolyze FPP into farnesol. Overexpression of FPP synthase (IspA) and PgpB, along with a heterologous mevalonate pathway, enabled recombinant E. coli to produce 526.1 mg/L of farnesol. This result indicates that the phosphatases PgpB and YbjG can be used to construct a novel farnesol synthesis pathway for mass production in E. coli.

ISSN 

1860-6768

Link 

http://dx.doi.org/10.1002/biot.201600250

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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