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Title 

Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration

Authors 

H KimD H KangK H KooS LeeS M KimJanghwan KimM H YoonS Y KimE G Yang

Publisher 

Royal Society of Chemistry

Issue Date 

2016

Citation 

Nanoscale, vol. 8, no. 42, pp. 18087-18097

Abstract 

The biological applications of vertical nanostructures mostly rely on their intracellular accessibility through the cellular membrane by promoting cell-to-nanostructure interactions. Herein, we report a seemingly counter-intuitive approach for the spontaneous formation of mouse induced pluripotent stem cell (iPSC)-derived three-dimensional spherical colonies with unlimited self-renewal and differentiation potential. The comprehensive analyses of iPSCs cultured on vertical silicon nanocolumn arrays (vSNAs) with various nanocolumn geometries show reduced cell-to-substrate adhesion and enhanced cell-to-cell interactions under optimized vSNA conditions, successfully accommodating the spontaneous production of iPSC-derived spherical colonies. Remarkably, these colonies which were only minimally penetrated by and thereby easily harvested from wafer-sized vSNAs display a substantial increase in pluripotency marker expression and successfully differentiate into three germ layers. Our vSNAs capable of large-scale fabrication, efficient for spherical colony formation, and reusable for multiple iPSC culture could serve as a broad-impact culture platform for stem cell research.

ISSN 

2040-3364

Link 

http://dx.doi.org/10.1039/c6nr06203b

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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