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Title 

Crystal structure of the cyan fluorescent protein Cerulean-S175G

 

시안 형광 단백질 Cerulean-S175G의 결정 구조

Authors 

Sang-wook ParkSunghyun KangTae-Sung Yoon

Publisher 

International Union of Crystallography

Issue Date 

2016

Citation 

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, vol. 72, no. 7, pp. 516-522

Keywords 

Cerulean-S175Genhanced cyan fluorescent proteinfluorescence lifetime imaging microscopyfluorescence resonance energy transferfluorophores

Abstract 

Enhanced cyan fluorescent protein (ECFP) was derived from Aequorea Victoria green fluorescent protein (avGFP), notably with S65T/Y66W mutations. Its chromophore consists of a tripeptide comprised of Thr65, Trp66 and Gly67 (TWG) residues, while that of avGFP consists of a Ser65, Tyr66 and Gly67 (SYG) tripeptide. Cerulean and SCFP3A were derived from ECFP-S72A/H148D (a double mutation) with additional Y145A and S175G mutations, respectively, while Cerulean-S175G has both mutations (Y145A and S175G). The crystal structures of these ECFP variants at neutral pH were reported to adopt two distinct major conformations called ECFP and Cerulean. In this study, Cerulean-S175G was revealed to adopt only the Cerulean conformation, while Cerulean has been reported to adopt both the ECFP and the Cerulean conformations in its crystal structures. Sharing the same S175G mutation with SCFP3A, Cerulean-S175G showed a slightly increased quantum yield, like SCFP3A, but did not adopt the ECFP conformation adopted by SCFP3A. Detailed comparison of Cerulean-S175G and other ECFP variants revealed that the notable conformational changes in ECFP variants can be understood mainly in terms of the interaction between the Trp66 residue of the chromophore and residues 145-148 of β-strand 7.

Citation 

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 72(7): 516-522

ISSN 

1744-3091

Link 

http://dx.doi.org/10.1107/S2053230X16008311

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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