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Title 

Structural investigation on the intrinsically disordered N-terminal region of HPV16 E7 protein

Authors 

Chewook LeeDo-Hyoung KimSi-Hyung LeeJ SuKyou Hoon Han

Publisher 

Korean Society for Biochemistry and Molecular Biology

Issue Date 

2016

Citation 

BMB Reports, vol. 49, no. 8, pp. 431-436

Keywords 

E7 oncoproteinHuman papillomavirus (HPV)Intrinsically disordered protein (IDP)Molecular dynamics (MD) simulationNuclear magnetic resonance (NMR)Pre-structured motif (PreSMo)

Abstract 

Human papillomavirus (HPV) is the major cause of cervical cancer, a deadly threat to millions of females. The early oncogene product (E7) of the high-risk HPV16 is the primary agent associated with HPV-related cervical cancers. In order to understand how E7 contributes to the transforming activity, we investigated the structural features of the flexible N-terminal region (46 residues) of E7 by carrying out N-15 heteronuclear NMR experiments and replica exchange molecular dynamics simulations. Several NMR parameters as well as simulation ensemble structures indicate that this intrinsically disordered region of E7 contains two transient (10-20% populated) helical pre-structured motifs that overlap with important target binding moieties such as an E2F-mimic motif and a pRb-binding LXCXE segment. Presence of such target-binding motifs in HPV16 E7 provides a reasonable explanation for its promiscuous target-binding behavior associated with its transforming activity.

ISSN 

1225-8687

Link 

http://dx.doi.org/10.5483/BMBRep.2016.49.8.021

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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