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Title 

Preparation of homogeneous solid samples for reproducible and quantitative MALDI

Authors 

S H AhnK M ParkJeong Hee MoonS H LeeM S Kim

Publisher 

The Korean Chemical Society

Issue Date 

2016

Citation 

Bulletin of the Korean Chemical Society, vol. 37, no. 0, pp. 458-462

Keywords 

MALDI quantificationMALDI temperatureMatrix suppressionMatrix-assisted laser desorption ionizationReproducible MALDI

Abstract 

Preparing samples with good homogeneity is one of the requirements for generating reproducible matrixassisted laser desorption ionization (MALDI) spectra and for using them in analyte quantification. Thus far, we have not been successful in producing homogeneous solid samples from popular matrixes apart from α-cyano-4-hydroxycinnamic acid (CHCA). In this work, we demonstrate the production of an outwardly homogeneous solid sample by loading a methanol solution of a matrix into a shallow reservoir on a coated MALDI sample plate and then vacuum-drying it. Out of ten popular matrixes tested, seven yielded homogeneous samples. These were 9-aminoacridine, 6-aza-2-thiothymine, CHCA, 2,5-dihydroxybenzoic acid, ferulic acid, sinapinic acid, and 2,4,6-trihydroxyacetophenone. For MALDI with these matrixes, linear calibration curves covering wide dynamic ranges were acquired. Performances of these matrixes in various aspects of analyte quantification have been investigated.

ISSN 

0253-2964

Link 

http://dx.doi.org/10.1002/bkcs.10704

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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