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Title 

A novel regulatory element (E77) isolated from CHO-K1 genomic DNA enhances stable gene expression in Chinese hamster ovary cells

Authors 

Shin-Young KangYeon-Gu KimSeunghee KangHong-Weon LeeEun Gyo Lee

Publisher 

Wiley-Blackwell

Issue Date 

2016

Citation 

Biotechnology Journal, vol. 11, no. 5, pp. 633-641

Keywords 

Chinese hamster ovary cellsCMV promoterGreen fluorescent proteinRegulatory DNA elementStable gene expression

Abstract 

Vectors flanked by regulatory DNA elements have been used to generate stable cell lines with high productivity and transgene stability; however, regulatory elements in Chinese hamster ovary (CHO) cells, which are the most widely used mammalian cells in biopharmaceutical production, are still poorly understood. We isolated a novel gene regulatory element from CHO-K1 cells, designated E77, which was found to enhance the stable expression of a transgene. A genomic library was constructed by combining CHO-K1 genomic DNA fragments with a CMV promoter-driven GFP expression vector, and the E77 element was isolated by screening. The incorporation of the E77 regulatory element resulted in the generation of an increased number of clones with high expression, thereby enhancing the expression level of the transgene in the stable transfectant cell pool. Interestingly, the E77 element was found to consist of two distinct fragments derived from different locations in the CHO genome shotgun sequence. High and stable transgene expression was obtained in transfected CHO cells by combining these fragments. Additionally, the function of E77 was found to be dependent on its site of insertion and specific orientation in the vector construct. Our findings demonstrate that stable gene expression mediated by the CMV promoter in CHO cells may be improved by the isolated novel gene regulatory element E77 identified in the present study

ISSN 

1860-6768

Link 

http://dx.doi.org/10.1002/biot.201500464

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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