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Title 

Tetanus toxin production from Clostridium tetani, using a casein-based medium in a single-use bioreactor

Authors 

Y J ChungM Y JungJ A LeeT Y KimYong Kyung ChoeI H Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2016

Citation 

Biotechnology and Bioprocess Engineering, vol. 21, no. 4, pp. 531-536

Keywords 

casein hydrolysateClostridium tetanisemisynthetic mediumsingle-use bioreactortetanus toxin

Abstract 

Clostridium tetani, a spore-forming anaerobic bacterium, and a casein-based semisynthetic medium were used to produce tetanus toxin in this study. N-Z-Case TT (casein hydrolysate) solution and glucose stock media were mixed and autoclaved, which resulted in tetanus toxin expression. The toxin was expressed when the N-Z-Case TT solution reacted with the glucose stock at a high temperature, creating an adequate amount of Maillard reaction products (MRPs). After accumulating in C. tetani cells, tetanus toxin was secreted into the medium when cell lysis was induced by surface aeration. C. tetani was cultivated and tetanus toxin was expressed in a single-use bioreactor, which produced 80 Lf/mL of tetanus toxin in a medium with MRPs. While using the correct medium to induce tetanus toxin was important, other factors played a part in achieving the desired concentration of the toxin, including the medium processing and culture methods inside the bioreactor. Tetanus toxoid with a purity level greater than 2,500 Lf/mgPN was obtained by detoxifying and purifying the toxin recovered from the fermenter or single-use bioreactor. A single-use bioreactor could be used in a limited space without the need for constructing a large scale production facility, to produce the tetanus toxoid antigen for clinical trials.

ISSN 

1226-8372

Link 

http://dx.doi.org/10.1007/s12257-016-0355-6

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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