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Title 

Osteoprotective effects of polysaccharide-enriched Hizikia fusiforme processing byproduct in vitro and in vivo models

Authors 

Y T JjeongS H BaekS C JeongYeo Dae YoonO H KimB C OhJ W JungJ H Kim

Publisher 

Mary Ann Liebert

Issue Date 

2016

Citation 

Journal of Medicinal Food, vol. 19, no. 8, pp. 805-814

Keywords 

Hizikia fusiformeosteoblastosteoclastovariectomized micezebrafish

Abstract 

The traditional manufacturing method used to produce goods from Hizikia fusiforme, utilizes extraction steps with hot water. The byproduct (of hot water extraction) is rich in polysaccharide and is considered a waste. To evaluate the osteogenic effects of the byproduct of H. fusiforme (HFB), osteogenic cells and animal models were used to test it effects on osteogenesis. The HFB-treated mouse myoblast C2C12 cells exhibited significant dose dependently elevated alkaline phosphatase (ALP) activity and slightly increased bone morphogenetic protein-2 (BMP-2). HFB also suppressed the formation of tartrate-resistant acid phosphatase (TRAP) activity and TRAP staining in the bone marrow-derived macrophages (BMM) cells that had been stimulated with the receptor activator of the nuclear factor kB ligand/macrophage colony-stimulating factor kB ligand. In addition, HFB also increased the phosphorylation of extracellular signal-regulated protein kinase (p-ERK) level. Finally, osteogenic effects of HFB were clearly confirmed in the three in vivo models: zebrafish, ovariectomized mice, and mouse calvarial bones. HFB accelerated the rate of skeletal development in zebrafish and prevented much of the mouse femoral bone density loss of ovariectomized mice. Moreover, HFB enhanced woven bone formation over the periosteum of mouse calvarial bones. Our result showed that HFB functions as a bone resorption inhibitor as well as an activator of bone formation in vivo and in osteogenic in vitro cell systems

ISSN 

1096-620X

Link 

http://dx.doi.org/10.1089/jmf.2015.3646

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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