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Title 

Effect of high hydrostatic pressure treatment on isoquercetin production from rutin by commercial α-L-rhamnosidase

 

엘 람노시다아제를 이용한 루틴으로부터 이소쿼세틴 생산성에 정수압이 미치는 영향

Authors 

D Y KimSoo Jin YeomC S ParkY S Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2016

Citation 

Biotechnology Letters, vol. 38, no. 0, pp. 1775-1780

Keywords 

Hydrostatic pressureIsoquercetinRutinTartary buckwheatα-l-rhamnosidase

Abstract 

Objectives: To optimize conversion of rutin to isoquercetin by commercial α-l-rhamnosidase using high hydrostatic pressure (HHP). Results: The de-rhamnosylation activity of α-l-rhamnosidase for isoquercetin production was maximal at pH 6.0 and 50?°C using HHP (150 MPa). The enzyme showed high specificity for rutin. The specific activity for rutin at HHP was 1.5-fold higher than that at atmospheric pressure. The enzyme completely hydrolysed 20 mM rutin in tartary buckwheat extract after 2 h at HHP, with a productivity of 10 mM h?1. The productivity and conversion were 2.2- and 1.5-fold higher at HHP than at atmospheric pressure, respectively. Conclusions: This is the first report concerning the enzymatic hydrolysis of isoquercetin in tartary buckwheat at HHP.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-016-2157-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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