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Title 

Higher production of C-phycocyanin by nitrogen-free (diazotrophic) cultivation of Nostoc sp. NK and simplified extraction by dark-cold shock

Authors 

Na Kyeong LeeHee-Mock OhHee-Sik KimChi-Yong Ahn

Publisher 

Elsevier

Issue Date 

2017

Citation 

Bioresource Technology, vol. 227, no. 0, pp. 164-170

Keywords 

Complementary chromatic adaptation (CCA)CyanobacteriaNitrogen fixationNostoc sp.Phycocyanin

Abstract 

Nostoc sp. NK (KCTC 12772BP) was isolated and cultivated in a BG11 medium and a nitrate-free BG11 medium (BG110). To enhance C-phycocyanin (C-PC) content in the cells, different fluorescent lamps (white, plant, and red) were used as light sources for complementary chromatic adaptation (CCA). The maximum biomass productivity was 0.42?g/L/d and 0.32?g/L/d under BG11 and BG110 conditions, respectively. The maximum C-PC contents were 8.4% (w/w) under white lamps, 13.6% (w/w) under plant lamps, and 18% (w/w) under BG110 and the red light condition. The maximum C-PC productivity was 57.4?mg/L/d in BG110 under the red lamp condition. These results indicate that a higher C-PC content could be obtained under a diazotrophic condition and a CCA reaction. The C-PC could be released naturally from cells without any extraction processes, when Nostoc sp. NK was cultivated in the BG110 medium with CO2 aeration and put in dark conditions at 5?°C.

ISSN 

0960-8524

Link 

http://dx.doi.org/10.1016/j.biortech.2016.12.053

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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