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Title 

Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

Authors 

J H KimC WangH J JangM S ChaJ E ParkS Y JoEui Sung ChoiS W Kim

Publisher 

BioMed Central

Issue Date 

2016

Citation 

Microbial Cell Factories, vol. 15, no. 0, pp. 214-214

Keywords 

BioisopreneCarbon utilizationEscherichia coliIsoprene synthaseMevalonate pathway

Abstract 

Background: Isoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives. Results: In this study, Escherichia coli MG1655 harboring Populus trichocarpa isoprene synthase (PtispS) and the exogenous mevalonate (MVA) pathway produced 80mg/L isoprene. Codon optimization and optimal expression of the ispS gene via adjustment of the RBS strength and inducer concentration increased isoprene production to 199 and 337mg/L, respectively. To augment expression of MVA pathway genes, the MVA pathway was cloned on a high-copy plasmid (pBR322 origin) with a strong promoter (Ptrc), which resulted in an additional increase in isoprene production up to 956mg/L. To reduce the formation of byproducts derived from acetyl-CoA (an initial substrate of the MVA pathway), nine relevant genes were deleted to generate the E. coli AceCo strain (E. coli MG1655 ?ackA-pta, poxB, ldhA, dld, adhE, pps, and atoDA). The AceCo strain harboring the ispS gene and MVA pathway showed enhanced isoprene production of 1832mg/L in flask culture with reduced accumulation of byproducts. Conclusions: We achieved a 23-fold increase in isoprene production by codon optimization of PtispS, augmentation of the MVA pathway, and deletion of genes involved in byproduct formation

ISSN 

1475-2859

Link 

http://dx.doi.org/10.1186/s12934-016-0612-6

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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