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Title 

Transcriptome profiling of sweetpotato tuberous roots during low temperature storage

Authors 

Chang Yoon JiW H ChungHo Soo KimWon Yong JungL KangJae Cheol JeongSang Soo Kwak

Publisher 

Elsevier

Issue Date 

2017

Citation 

Plant Physiology & Biochemistry

Keywords 

Chilling injuryDifferentially expressed genesLow temperature storageSweetpotatoTranscriptome

Abstract 

Sweetpotato [Ipomoea batatas (L.) Lam] is a globally important root crop with high industrial value. However, because sweetpotato tuberous roots undergo chilling injuries that negatively affect their quality at temperatures below 10?°C, postharvest damage during the winter season is a major constraint for industrialization. To understand chilling injury response during postharvest low temperature storage, we used next-generation sequencing technology to comprehensive analyze the transcriptome of tuberous roots stored at optimal (13?°C) or low temperature (4?°C) for 6 weeks. From nine cDNA libraries, we produced 298,765,564 clean reads, which were de novo assembled into 58,392 unigenes with an average length of 1100 bp. A total of 3216 differentially expressed genes (DEGs) were detected and categorized into six clusters, of which clusters 2, 4, and 5 (1464 DEGs) were up-regulated under low temperature. The genes in these three clusters are involved in biosynthesis of unsaturated fatty acids, pathogen defense, and phenylalanine metabolism. By contrast, genes in clusters 1, 3, and 6 (1752 DEGs), which were generally down-regulated at low temperature, encode antioxidant enzymes or are involved in glycerophospholipid, carbohydrate, or energy metabolism. We confirmed the results of the transcriptome analysis by quantitative RT-PCR. Our transcriptome analysis will advance our understanding of the comprehensive mechanisms of chilling injury during low temperature storage and facilitate improvements in postharvest storage of sweetpotato tuberous roots

ISSN 

0981-9428

Link 

http://dx.doi.org/10.1016/j.plaphy.2016.12.021

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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