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Title 

Alginate/PEI/DNA polyplexes: a new gene delivery system

Authors 

G JiangSang-Hyun MinMi-Na KimDong Chul LeeMi-Jung LimYoung Il Yeom

Publisher 

Nature Publishing Group

Issue Date 

2006

Citation 

Acta Pharmacologica Sinica

Keywords 

AlginateGene deliveryPolyethyleniminePolyplex

Abstract 

Aim: To avoid the limitation of the use of cationic polyethlenimine (PEI)-complexed plasmid DNA use for in vitro or in vivo gene delivery due to its cytotoxicity and lower efficiency in the presence of serum. Methods: A polyplex with decreased positive charge on the complex surface was designed. The PEI/DNA (PD) complexes coated with an anionic biodegradable polymer, alginate were prepared and their gene delivery behavior with PD was compared. Results: The alginate-coated PD polyplex, where alginate: PEI: DNA [alginate: DNA, 0.15 (w/w); PEI: DNA, N:P = 10] showed about 10-30 fold-increased transfection efficiency compared to corresponding non-coated complexes to C3 cells in the presence of 50% serum. The surface charge of the alginate-coated complex was approximately half of that of the alginate-lacking complex. The size of alginate-coated complex was slightly smaller than that of the corresponding complex without alginate. The former complex also showed a reduced erythrocyte aggregation activity and decreased cytotoxicities to C3 cells in comparison with PD complex. Conclusion: The alginate-coated PD polyplexes as a new gene delivery system can improve transfection efficiency in high serum concentration with low cytotoxicity to C3 cells.

ISSN 

1671-4083

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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