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Title 

In vivo imaging of β-galactosidase stimulated activity in hepatocellular carcinoma using ligand-targeted fluorescent probe

Authors 

E J KimR KumarA SharmaB YoonHyun Min KimHyunseung LeeKwan Soo HongJ S Kim

Publisher 

Elsevier

Issue Date 

2017

Citation 

Biomaterials

Keywords 

Fluorescent probeHepatocellularIn vivo imagingRatiometricβ-galactosidase

Abstract 

Development of targeted, selective, and noninvasive fluorescent probes for in?vivo visualization of tumor-associated overexpressed enzymes are highly anticipated for cancer diagnosis and therapy. Herein, we developed a noninvasive fluorescent probe (DCDHF-βgal) for the sensitive detection, and in?vivo visualization of β-galactosidase in hepatocyte HepG2 cells and its xenograft model. As a model system for in?vivo targeted imaging, DCDHF-βgal possessing galactose unit selectively target hepatocyte and monitor the β-galactosidase activity with deep tissue penetration, and low background interference. DCDHF-βgal was activated by intracellular β-galactosidases as the driving force for the release of NIR fluorophore, thereby exhibiting ratiometric optical response. Initial fluorescence emission measured at 615?nm was changed to fluorescence at 665?nm upon activation of DCDHF-βgal with β-galactosidase. Ratiometric fluorescence detection of β-galactosidase was also observed in hepatocellular carcinoma cells and tumor xenograft. The noninvasive in?vivo optical imaging facilitated by targeted and enzyme-activated imaging agent would be useful in various biomedical and diagnostic applications.

ISSN 

0142-9612

Link 

http://dx.doi.org/10.1016/j.biomaterials.2017.01.009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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