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Title 

Establishment and characterization of immortalized minipig neural stem cell line

 

불멸화 미니돼지 신경줄기세포주의 확립 및 특성 분석

Authors 

S S ChoiSeung-Bin YoonSang-Rae LeeSun-Uk KimY J ChaD LeeS U KimKyu Tae ChangH J Lee

Publisher 

Cognizant Communication Corporation

Issue Date 

2017

Citation 

Cell Transplantation

Keywords 

ImmortalizationMinipigNeural stem cells (NSCs)Olfactory bulbv-myc

Abstract 

Despite the increasing importance of minipigs in biomedical research, there has been relatively little research concerning minipig-derived adult stem cells as a promising research tool that could be used to develop stem cell-based therapies. We first generated immortalized neural stem cells (iNSCs) from primary minipig olfactory bulb cells (pmpOBCs) and defined the characteristics of the cell line. Primary neural cells were prepared from minipig neonate olfactory bulbs and immortalized by infection with retrovirus carrying the v-myc gene. The minipig iNSCs (mpiNSCs) had normal karyotypes and expressed NSC-specific markers, including nestin, vimentin, Musashi1, and SOX2, suggesting a similarity to human NSCs. On the basis of the global gene expression profiles from the microarray analysis, neurogenesis-associated transcript levels were predominantly altered in mpiNSCs compared with pmpOBCs. These findings increase our understanding of minipig stem cells and contribute to the utility of mpiNSCs as resources for immortalized stem cell experiments.

ISSN 

0963-6897

Link 

http://dx.doi.org/10.3727/096368916X692852

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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