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Title 

Alu-derived alternative splicing events specific to Macaca lineages in CTSF gene

Authors 

Ja-Rang LeeSang Je ParkYoung-Hyun KimSe Hee ChoeHyeon-Mu ChoSang-Rae LeeSun-Uk KimJi-Su KimBo Woong SimBong-Seok SongKang Jin JeongYoungjeon LeeYeung Bae JinPhil Yong KangJae Won HuhKyu Tae Chang

Publisher 

Springer Verlag (Germany)

Issue Date 

2017

Citation 

Molecules and Cells

Keywords 

Alternative splicing AluCTSFExonizationPrimate

Abstract 

Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element?AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonization events in the CTSF gene of human, rhesus monkey, and crabeating monkey were investigated using PCR and reverse transcription PCR on the genomic DNA and cDNA isolated from several tissues. Results demonstrated that AluYRa1 was only integrated into the genome of Macaca species and this lineage- specific integration led to exonization events by producing a conserved 3’ splice site. Six transcript variants (V1?V6) were generated by alternative splicing (AS) events, including intron retention and alternative 5’ splice sites in the 5’ and 3’ flanking regions of CTSF_AluYRa1. Among them, V3?V5 transcripts were ubiquitously expressed in all tissues of rhesus monkey and crab-eating monkey, whereas AluYRa1-exonized V1 was dominantly expressed in the testis of the crab-eating monkey, and V2 was only expressed in the testis of the two monkeys. These five transcript variants also had different amino acid sequences in the C-terminal region of CTSF, as compared to reference sequences. Thus, species-specific Aluderived exonization by lineage-specific integration of Alu elements and AS events seems to have played an important role during primate evolution by producing transcript variants and gene diversification.

ISSN 

1016-8478

Link 

http://dx.doi.org/10.14348/molcells.2017.2204

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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