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Title 

In silico study on Arabidopsis BAG gene expression in response to environmental stresses

Authors 

G M NawkarP MaibamJ H ParkWoo Su GyeongCha Young KimS Y LeeC H Kang

Publisher 

Springer Verlag (Germany)

Issue Date 

2017

Citation 

Protoplasma

Keywords 

Bcl-2 athanogene (BAG)Environmental stressIn silico dataStress-responsive elementsTranscription profiling

Abstract 

BAG (Bcl-2 athanogene) family proteins are conserved in a wide range of eukaryotes, and they have been proposed to play a crucial role in plant programmed cell death (PCD). During the past decade, with the help of advanced bioinformatics tools, seven homologs of BAG genes have been identified in the Arabidopsis genome; these genes are involved in pathogen attack and abiotic stress conditions. In this study, gene expression of Arabidopsis BAG family members under environmental stresses was analyzed using the Botany Array Resource (BAR) expression browser tool and the in silico data were partially confirmed by qRT-PCR analysis for the selected stress- and hormone-treated conditions related to environmental stresses. Particularly, the induction of AtBAG6 gene in response to heat shock was confirmed by using GUS reporter lines. The loss of the AtBAG6 gene resulted into impairment in basal thermotolerance of plant and showed enhanced cell death in response to heat stress. To elucidate the regulatory mechanisms of BAG genes, we analyzed ∼1-kbp promoter regions for the presence of stress-responsive elements. Our transcription profiling finally revealed that the Arabidopsis BAG genes differentially respond to environmental stresses under the control of specifically organized upstream regulatory elements.

ISSN 

0033-183X

Link 

http://dx.doi.org/10.1007/s00709-016-0961-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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