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Title 

Kahweol induces apoptosis by suppressing BTF3 expression through the ERK signaling pathway in non-small cell lung cancer cells

Authors 

Y J JeonW BangJ H ChoR H LeeSeok Ho KimM S KimS M ParkJ C ShinH J ChungK B OhJ M SeoS KoJ H ShimJ I Chae

Publisher 

Spandidos Publications

Issue Date 

2016

Citation 

International Journal of Oncology

Keywords 

ApoptosisBasic transcription factor 3ERKKahweolNon-small cell lung cancer

Abstract 

Kahweol, a diterpene molecule, has antiproliferative effects on several types of human cancer cells, but whether it has apoptotic effect in non-small cell lung cancer (NSCLC) is not known. To explore this possibility, we incubated cells from two NSCLC cell lines, NCI-H358 and NCI-H1299, with different concentrations of kahweol and used the MTS assay, DAPI staining, propidium iodide staining, Annexin V staining, immunocytochemical test, and western blot analysis to characterize this molecule and the signaling pathway underlying its effects. The kahweol-treated cells showed significantly decreased cell viability, increased nuclear condensation, and an increased number of Annexin V-positive NSCLC cells. Suppression of basic transcription factor 3 (BTF3) was followed by apoptosis induced by kahweol via the ERK-mediated signaling pathway in a dose- and time-dependent manner. In addition, kahweol modulated the protein expression of BTF3 genes involved in cell-cycle regulation and apoptosis-related proteins, resulting in apoptotic cell death. Our results collectively indicated that kahweol inhibited the proliferation of NSCLC cells through ERK-mediated signaling pathways and the downregulation of BTF3.

ISSN 

1019-6439

Link 

http://dx.doi.org/10.3892/ijo.2016.3727

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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