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Title 

Suppressing mosaicism by Au nanowire injector-driven direct delivery of plasmids into mouse embryos

Authors 

K ParkK C KimH LeeY SungM KangY M LeeJ Y AhnJ M LimTaejoon KangB KimE J Lee

Publisher 

Elsevier

Issue Date 

2017

Citation 

Biomaterials

Keywords 

Gene deliveryGold nanowireMosaicismNanoinjectorTransgenic animal

Abstract 

Transgenic animals have become key tools in a variety of biomedical research areas. However, microinjection commonly used for producing transgenic animals has several challenges such as physical and chemical damage to the embryos due to microinjector with buffer, and low transgene integration rates with frequent mosaicism. Here, we report direct delivery of plasmids into mouse embryos using a Au nanowire injector (NWI) that significantly improved transgene integration efficiency and suppressed mosaicism. The Au NWI could deliver plasmid into the pronucleus (PN) of a mouse zygote without buffer and rapidly release it with electric pulse. Because zygote, which is a fertilized 1-cell stage embryo, has two physical barriers (cytoplasmic membrane and zona pellucida), direct delivery of plasmids into PN of zygote is more difficult than into a normal cell type. To penetrate the two physical barriers with minimal disruption of the embryo, we optimized the diameter and length of Au NWI. The mosaicism is more reduced in the Au NWI injected embryos than in micropipette injected embryos, which was determined by the expression of transgene in a blastocyst stage. We suggest that Au NWI can increase the efficiency of gene delivery into zygote with suppressed mosaicism and become a useful alternative

ISSN 

0142-9612

Link 

http://dx.doi.org/10.1016/j.biomaterials.2017.05.044

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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