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Title 

Biological and chemical strategies for exploring inter- and intra-kingdom communication mediated via bacterial volatile signals

Authors 

M A FaragGeun Cheol SongYong-Soon ParkB AudrainSoohyun LeeJ M GhigoJ W KloepperChoong-Min Ryu

Publisher 

Nature Publishing Group

Issue Date 

2017

Citation 

Nature Protocols

Abstract 

Airborne chemical signals emitted by bacteria influence the behavior of other bacteria and plants. We present an overview of in vitro methods for evaluating bacterial and plant responses to bacterial volatile compounds (BVCs). Three types of equipment have been used to physically separate the bacterial test strains from either other bacterial strains or plants (in our laboratory we use either Arabidopsis or tobacco plant seedlings): a Petri dish containing two compartments (BI Petri dish); two Petri dishes connected with tubing; and a microtiter-based assay. The optimized procedure for the BI Petri dish system is described in this protocol and can be widely used for elucidation of potential function in interactions between diverse microbes and those plant and chemical volatiles emitted by bacteria that are most likely to mediate bacterial or plant responses to BVCs. We also describe a procedure for metabolome-based BVC profiling via dynamic (i.e., continuous airflow) or static headspace sampling using solid-phase microextraction (SPME). Using both these procedures, bacteria-bacteria communications and bacteria-plant interactions mediated by BVCs can be rapidly investigated (within 1-4 weeks).

ISSN 

1754-2189

Link 

http://dx.doi.org/10.1038/nprot.2017.023

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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