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Title 

The mechanism of starch over-accumulation in Chlamydomonas reinhardtii high-starch mutants identified by comparative transcriptome analysis

Authors 

K M KooS JungB S LeeJ B KimY D JoH I ChoiS Y KangG H ChungWon Joong JeongJ W Ahn

Publisher 

Frontiers Research Foundation

Issue Date 

2017

Citation 

Frontiers in Microbiology

Keywords 

Chlamydomonas reinhardtiiComparative transcriptome analysisGlycolysisMicroalgaeStarch biosynthesis

Abstract 

The focus of this study was the mechanism of starch accumulation in Chlamydomonas reinhardtii high-starch mutants. Three C. reinhardtii mutants showing high-starch content were generated using gamma irradiation. When grown under nitrogen-deficient conditions, these mutants had more than twice as much starch than a wild-type control. The mechanism of starch over-accumulation in these mutants was studied with comparative transcriptome analysis. In all mutants, induction of phosphoglucomutase 1 (PGM1) expression was detected; PGM1 catalyzes the inter-conversion of glucose 1-phosphate and glucose 6-phosphate in both starch biosynthetic and glycolytic pathway. Interestingly, transcript levels of phosphoglucose isomerase 1 (PGI1), fructose 1,6-bisphosphate aldolase 1 and 2 (FBA1 and FBA2) were down-regulated in all mutants; PGI1, FBA1, and FBA2 act on downstream of glucose 6-phosphate conversion in glycolytic pathway. Therefore, down-regulations of PGI1, FBA1, and FBA2 may lead to accumulation of upstream metabolites, notably glucose 6-phosphate, resulting in induction of PGM1 expression through feed-forward regulation and that PGM1 overexpression caused starch over-accumulation in mutants. These results suggest that PGI1, FBA1, FBA2, and PGM1 correlate with each other in terms of coordinated transcriptional regulation and play central roles for starch over-accumulation in C. reinhardtii.

ISSN 

1664-302x

Link 

http://dx.doi.org/10.3389/fmicb.2017.00858

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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