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Title 

Optimization of herbicidin a production in submerged culture of streptomyces scopuliridis M40

Authors 

S HaK J LeeS I LeeH J GwakJ H LeeT W KimH J ChoiJ Y JangJ S ChoiChang-Jin KimJ C KimH H KimH W Park

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2017

Citation 

Journal of Microbiology and Biotechnology

Keywords 

Herbicidin AHyphal lengthOxygen uptake rateScale-upStreptomyces scopuliridis M40

Abstract 

Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of Streptomyces scopuliridis M40 were investigated. The herbicidin A concentration varied with the C/N ratio. High C/N ratios (>4) resulted in a herbicidin A production of more than 900 mg/l, whereas maximally 600 mg/l was obtained at ratios between 1 and 3.5. In 5-L batch fermentation, there was a positive correlation between the oxygen uptake rate (OUR) and herbicidin A production. Once the OUR increased, the substrate consumption rate increased, leading to an increase in volumetric productivity. Mechanical shear force affected the hyphal morphology and OUR. When the medium value of hyphal size ranged from 150 to 180 ?m, high volumetric production of herbicidin A was obtained with OUR values >137 mg O2 /l·h. The highest herbicidin A concentration of 956.6 mg/l was obtained at 500 rpm, and coincided with the highest relative abundance of2 hyphae of 100?200 ?m length and the highest OUR during cultivation. Based on a constant impeller tip speed, which affects hyphal morphology, herbicidin A production was successfully scaled up from a 5-L jar to a 500-L pilot vessel.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1611.11005

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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