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Title 

Profiling of cytosolic and mitochondrial H2O2 production using the H2O2-sensitive protein HyPer in LPS-induced microglia cells

Authors 

Junghyung ParkS LeeH S LeeSang-Rae LeeD S Lee

Publisher 

Elsevier

Issue Date 

2017

Citation 

Neuroscience Letters

Keywords 

Hydrogen peroxideHyPerLipopolysaccharideMicrogliaMitogen-activated protein kinaseReactive oxygen species

Abstract 

Dysregulation of the production of pro-inflammatory mediators in microglia exacerbates the pathologic process of neurodegenerative disease. ROS actively affect microglia activation by regulating transcription factors that control the expression of pro-inflammatory genes. However, accurate information regarding the function of ROS in different subcellular organelles has not yet been established. Here, we analyzed the pattern of cytosolic and mitochondrial H2O2 formation in LPS-activated BV-2 microglia using the H2O2-sensitive protein HyPer targeted to specific subcellular compartments. Our results show that from an early time, cytosolic H2O2 started increasing constantly, whereas mitochondrial H2O2 rapidly increased later. In addition, we found that MAPK affected cytosolic H2O2, but not mitochondrial H2O2. Consequently, our study provides the basic information about subcellular H2O2 generation in activated microglia, and a useful tool for investigating molecular targets that can modulate neuroinflammatory responses.

ISSN 

0304-3940

Link 

http://dx.doi.org/10.1016/j.neulet.2017.06.017

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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