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Title 

Iloprost supports early development of in vitro-produced porcine embryos through activation of the phosphatidylinositol 3-kinase/AKT signalling pathway

Authors 

Pil Soo JeongSeung-Bin YoonSeon A ChoiBong-Seok SongJi-Su KimBo Woong SimYoung-Ho ParkHae Jun YangSeong-Eun MunYoung-Hyun KimPhilyong KangKang Jin JeongYoungjeon LeeYeung Bae JinJae Won HuhSang-Rae LeeD B KooY I ParkSun-Uk KimKyu Tae Chang

Publisher 

CSIRO Publishing

Issue Date 

2017

Citation 

Reproduction Fertility and Development

Keywords 

culture mediumsignal transduction

Abstract 

Despite evidence of the presence of prostaglandin (PG) I2 in mammalian oviducts, its role in early development of in vitro-produced (IVP) embryos is largely unknown. Thus, in the present study we examined the effects of iloprost, a PGI2 analogue, on the in vitro developmental competence of early porcine embryos and the underlying mechanism(s). To examine the effects of iloprost on the development rate of IVF embryos, iloprost was added to the in vitro culture (IVC) medium and cultured for 6 days. Supplementation of the IVC medium with iloprost significantly improved developmental parameters, such as blastocyst formation rate, the trophectoderm:inner cell mass ratio and cell survival in IVF and parthenogenetically activated (PA) embryos. In addition, post-blastulation development into the expanded blastocyst stage was improved in iloprost-treated groups compared with controls. Interestingly, the phosphatidylinositol 3-kinase (PI3K)/AKT signalling pathway was significantly activated by iloprost supplementation in a concentration-dependent manner (10-1000nM), and the beneficial effects of iloprost on the early development of porcine IVF and PA embryos was completely ablated by treatment with 2.5M wortmannin, a PI3K/AKT signalling inhibitor. Importantly, expression of the PI3K/AKT signalling pathway was significantly reduced in somatic cell nuclear transfer (SCNT) compared with IVF embryos, and iloprost supported the early development of SCNT embryos, as was the case for IVF and PA embryos, suggesting a consistent effect of iloprost on the IVC of IVP porcine embryos. Together, these results indicate that iloprost can be a useful IVC supplement for production of IVP early porcine embryos with high developmental competence

ISSN 

1031-3613

Link 

http://dx.doi.org/10.1071/RD15391

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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