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Title 

Enhancement of 2,3-butanediol production from Jerusalem artichoke tuber extract by a recombinant Bacillus sp. strain BRC1 with increased inulinase activity

 

바실러스 BRC1을 이용한 돼지감자로부터 2,3-부탄디올 생산

Authors 

Jang Min ParkBaek Rock OhIn Yeong KangSun-Yeon HeoJeong-Woo SeoS M ParkW K HongChul Ho Kim

Publisher 

Springer

Issue Date 

2017

Citation 

Journal of Industrial Microbiology (& Biotechnology)

Keywords 

2,3-ButanediolBacillus speciesFructan hydrolysisJerusalem artichoke tubersLevanase

Abstract 

A Bacillus sp. strain named BRC1 is capable of producing 2,3-butanediol (2,3-BD) using hydrolysates of the Jerusalem artichoke tuber (JAT), a rich source of the fructose polymer inulin. To enhance 2,3-BD production, we undertook an extensive analysis of the Bacillus sp. BRC1 genome, identifying a putative gene (sacC) encoding a fructan hydrolysis enzyme and characterizing the activity of the resulting recombinant protein expressed in and purified from Escherichia coli. Introduction of the sacC gene into Bacillus sp. BRC1 using an expression vector increased enzymatic activity more than twofold. Consistent with this increased enzyme expression, 2,3-BD production from JAT was also increased from 3.98 to 8.10 g L?1. Fed-batch fermentation of the recombinant strain produced a maximal level of 2,3-BD production of 28.6 g L?1, showing a high theoretical yield of 92.3%.

ISSN 

0169-4146

Link 

http://dx.doi.org/10.1007/s10295-017-1932-1

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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