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Title 

Characterisation of conformational and functional features of alkyl hydroperoxide reductase E-like protein

Authors 

S LeeH JeongJu Huck LeeJ M ChungR KimH J YunJ WonH S Jung

Publisher 

Elsevier

Issue Date 

2017

Citation 

Biochemical and Biophysical Research Communications

Keywords 

AhpEDNA binding proteinHoldasePeroxidaseStructure-function relationshipThermococcus kodakarensis

Abstract 

Alkyl hydroperoxide reductase E (AhpE) is a member of the peroxidase family of enzymes that catalyse the reduction of peroxides, however its structural and functional roles are still unclear in details. In this study, we used the Thermococcus kodakarensis AhpE-like protein as a model to investigate structure?function relationships including the molecular properties of DNA binding activity. Multiple sequence alignment, structural comparison and biochemical analyses revealed that TkAhpE includes conserved peroxidase residues in the active site, and exhibits peroxidase activity with structure-dependent holdase chaperone function. Following electrophoretic mobility shift assays and electron microscopy analysis demonstrated distinctive binding features of TkAhpE to the DNA showing that their dimeric conformer can bind to the double-stranded DNA, but not to the single-stranded DNA, indicating its striking molecular features to double-stranded DNA-specific interactions. Based on our results, we provided that TkAhpE is a multifunctional peroxidase displaying structure-dependent molecular chaperone and DNA binding activities.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2017.05.135

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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