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Title 

Activity analysis of LTR12C as an effective regulatory element of the RAE1 gene

Authors 

Yi Deun JungH E LeeA JoI HirooH J ChaH S Kim

Publisher 

Elsevier

Issue Date 

2017

Citation 

Gene

Keywords 

Long terminal repeatNuclear transcription factor YRegulatory elementRibonucleic acid export 1 (RAE1)

Abstract 

Ribonucleic acid export 1 (RAE1) plays an important role in the export of mature mRNAs from the nucleus to the cytoplasm. Long terminal repeats (LTRs) became integrated into the human genome during primate evolution. One such repeat element, LTR12C, lies within a predicted regulatory region located upstream of the RAE1 gene. We examined the transcriptional activity of LTR12C by using the luciferase assay, and showed that the tandem repeat region (TRR) located within LTR12C was required for its regulatory function. A bioinformatics analysis revealed that the LTR12C element had multiple transcription factor binding sites specific for nuclear transcription factor Y (NF-Y), and the promoter activity of LTR12C was significantly decreased after NF-Y knockdown. Additionally, we discovered novel data indicating that LTR12C was initially inserted into the gorilla genome. Taken together, our results reveal that the TRR of LTR12C has powerful regulatory activity due to its NF-Y binding sites, and the integration of the LTR12C element into the primate genome during evolution may have affected RAE1 transcription

ISSN 

0378-1119

Link 

http://dx.doi.org/10.1016/j.gene.2017.08.037

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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