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Title 

Native plants (Phellodendron amurense and Humulus japonicus) extracts act as antioxidants to support developmental competence of bovine blastocysts

Authors 

G Y DoJ W KimH J ParkSeung-Bin YoonJ Y ParkS G YangB D JungY S KwonM J KangBong-Seok SongSun-Uk KimKyu Tae ChangD B Koo

Publisher 

The Asian-australasian Association of Animal

Issue Date 

2017

Citation 

Asian-Australasian Journal of Animal Sciences

Keywords 

AntioxidantsBovine EmbryoHumulus japonicasPhellodendron amurenseReactive Oxygen Species (ROS)

Abstract 

Objective: Phellodendron amurense (P. amurense) and Humulus japonicus (H. japonicus) are closely involved in anti-oxidative response and increasing antioxidant enzymes activities. However, the effects of their extracts on development of preimplantation bovine embryos have not been investigated. Therefore, we investigated the effects of P. amurense and H. japonicus extracts on developmental competence and quality of preimplantation bovine embryos. Methods: After in vitro fertilization, bovine embryos were cultured for 7 days in Charles Rosenkrans amino acid medium supplemented with P. amurense (0.01 μg/mL) and H. japonicus (0.01 μg/mL). The effect of this supplementation during in vitro culture on development competence and antioxidant was investigated. Results: We observed that the blastocysts rate was significantly increased (p<0.05) in P. amurense (28.9%±2.9%), H. japonicus (30.9%±1.5%), and a mixture of P. amurense and H. japonicus (34.8%± 2.1%) treated groups compared with the control group (25.4%±1.6%). We next confirmed that the intracellular levels of reactive oxygen species (ROS) were significantly decreased (p<0.01) in P. amurense and/or H. japonicus extract treated groups when compared with the control group. Our results also showed that expression of cleaved caspase-3 and apoptotic cells of blastocysts were significantly decreased (p<0.05) in bovine blastocysts derived from both P. amurense and H. japonicus extract treated embryos. Conclusion: These results suggest that proper treatment with P. amurense and H. japonicus extracts in the development of preimplantation bovine embryos improves the quality of blastocysts, which may be related to the reduction of ROS level and apoptosis

ISSN 

1011-2367

Link 

http://dx.doi.org/10.5713/ajas.16.0985

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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