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Title 

Changes in epigenetic markers, DNMT1 and HDAC1/2, in the adult mouse hippocampus after cranial irradiation

Authors 

S KangYeonghoon SonS LeeJ KimJ C KimJ S KimU JungS H KimM YangC Moon

Publisher 

Elsevier

Issue Date 

2017

Citation 

Neuroscience Letters

Keywords 

Cranial irradiationDNA methyltransferaseEpigenetic modificationHippocampusHistone deacetylase

Abstract 

Brain exposure to ionizing radiation can cause functional deficits in the hippocampus, including memory impairment. However, the specific molecular mechanisms underlying irradiation-induced cognitive impairments are largely unknown. Changes in DNA methyltransferases (DNMTs) and histone deacetylases (HDACs), which are involved in DNA methylation and histone remodeling, may be associated with behavioral changes in learning and memory. We assessed changes in the levels of enzymes associated with the epigenetic modification of gene expression, including DNMT1, HDAC1, HDAC2, Sirtuin 1 (SIRT1), and acetylated histone H3 (Ace-H3) in the mouse hippocampus 1 and 30 days after a single exposure to cranial irradiation (0 or 10 Gy). mRNA levels of HDAC1 were significantly downregulated 1 day after irradiation with 10 Gy, and those of DNMT1, HDAC1, and HDAC2 were significantly downregulated 30 days post-irradiation. Western blot analysis revealed significant decreases in DNMT1, HDAC1, and HDAC2 protein levels 1 and 30 days after irradiation with 10 Gy. Furthermore, protein levels of SIRT1 and Ace-H3 were significantly downregulated in the mouse hippocampus 1 and 30 days after cranial irradiation. Our findings suggest that the reduction in epigenetic gene expression is associated with hippocampal dysfunction in mice exposed to cranial irradiation

ISSN 

0304-3940

Link 

http://dx.doi.org/10.1016/j.neulet.2017.08.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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