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Title 

Methylation levels of LINE-1 as a useful marker for venous invasion in both FFPE and frozen tumor tissues of gastric cancer

Authors 

J MinB ChoiTae Su HanH J LeeS H KongY S SuhT H KimH N KimW H KimK HurH K Yang

Publisher 

Springer Verlag (Germany)

Issue Date 

2017

Citation 

Molecules and Cells

Keywords 

Gastric cancerLINE-1PyrosequencingVenous invasion

Abstract 

Long interspersed nuclear element-1 (LINE-1) is a retrotransposon that contains a CpG island in its 5'-untranslated region. The CpG island of LINE-1 is often heavily methylated in normal somatic cells, which is associated with poor prognosis in various cancers. DNA methylation can differ between formalin-fixed paraffin-embedded (FFPE) and frozen tissues. Therefore, this study aimed to compare the LINE-1 methylation status between the two tissue-storage conditions in gastric cancer (GC) clinical samples and to evaluate whether LINE-1 can be used as an independent prognostic marker for each tissue-storage type. We analyzed four CpG sites of LINE-1 and examined the methylation levels at these sites in 25 FFPE and 41 frozen GC tissues by quantitative bisulfite pyrosequencing. The LINE-1 methylation status was significantly different between the FFPE and frozen GC tissues (p < 0.001). We further analyzed the clinicopathological features in the two groups separately. In the frozen GC tissues, LINE-1 was significantly hypomethylated in GC tissues compared to their corresponding normal gastric mucosa tissues (p < 0.001), and its methylation status was associated with gender, differentiation state, and lymphatic and venous invasion of GC. In the FFPE GC tissues, the methylation levels of LINE-1 differed according to tumor location and venous invasion of GC. In conclusion, LINE-1 can be used as a useful methylation marker for venous invasion in both FFPE and frozen tumor tissues of GC.

ISSN 

1016-8478

Link 

http://dx.doi.org/10.14348/molcells.2017.0013

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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